IsoPS-DIA: Dual Functionality of Absolute Targeted Quantification and Global Proteome Profiling.

ABSTRACT: Current gene testing reveals only the mutation status yet lacks protein expression of the actual drug target. A comprehensive evaluation requires methods that integrate the absolute quantification of mutant proteins with global profiling of downstream signaling and resistance pathways. Here, we present an isotope pair-separated data-independent acquisition (IsoPS-DIA) strategy with a dual functionality of multiplexed absolute quantification and global proteome profiling in a single run. IsoPS-DIA features a dual-window design: narrow consecutive windows separate light/heavy-isotope-labeled peptide pairs to reduce coisolation interference and maximize usable fragment ions, while wide variable windows capture proteome-wide information. Using EGFR mutations (L858R, G719A, Del19) in lun

SUBMITTER: Chan HJ 

PROVIDER: S-EPMC12937053 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature