Electrophoresis: An Effective Viability Assessment Method across Cell Domains.

ABSTRACT: Cell viability assessments are a critical step in numerous research, clinical and drug development processes. Electrokinetic techniques, such as electrorotation and dielectrophoresis, have shown promise as viability assessment methods, but often under specific and limited conditions. This study explores insulator-based electrokinetic (iEK) systems as a promising analytical alternative, leveraging differences in electromigration to rapidly separate viable and nonviable cells in a microfluidic system. To demonstrate broad efficacy and domain-agnostic discrimination, two prokaryotic bacterial lines (Escherichia coli and Salmonella Typhimurium) and two eukaryotic yeast strains (Saccharomyces cerevisiae ATCC 4098 and ATCC 9080) were studied. This work expands upon previous

SUBMITTER: Espino J 

PROVIDER: S-EPMC12937056 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature