FANS uncovers WOX2-associated early regulators of auxin-induced somatic embryogenesis in Arabidopsis.

ABSTRACT: Somatic embryogenesis (SE), the process by which differentiated somatic cells are reprogrammed to form embryos, represents a unique manifestation of plant cell totipotency. Despite its fundamental and applied importance, the molecular mechanisms that initiate embryogenic reprogramming remain largely unknown, mainly because explant tissues are cell-type heterogeneous and contain only a small fraction of SE-competent cells. Here, we applied fluorescence-activated nuclei sorting (FANS) in Arabidopsis thaliana to isolate cells expressing the WOX2 gene, a marker for early embryogenesis. Comparative transcriptomic analysis of WOX2⁽⁺⁾-positive and WOX2^(-)-negative nuclei revealed that genes up-regulated in the embryogenic nuclei were strongly enriched in biological processes

SUBMITTER: Wojcik AM 

PROVIDER: S-EPMC12937515 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature