Rapid Forensic DNA Profiling via Real-Time Recombinase Polymerase Amplification of InDel Markers.

ABSTRACT: Forensic DNA profiling commonly relies on polymerase chain reaction (PCR) amplification followed by capillary electrophoresis (CE) or massively parallel sequencing (MPS), which requires expensive, laboratory-based equipment that depends on a stable power supply and is unsuitable for field applications. Here, we present a proof-of-concept assay that uses recombinase polymerase amplification (RPA) combined with exo probe detection for rapid, isothermal genotyping of insertion-deletion (InDel) markers. To the best of our knowledge, this study represents the first demonstration of forensic DNA typing using RPA coupled with exo probes. The reaction proceeds at 39 °C and combines amplification and detection in a single 20 min step. Thirteen DNA samples were genotyped in triplicate across eight I

SUBMITTER: De Keyzer L 

PROVIDER: S-EPMC12938080 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature