Simplified Sample Preparation and Lateral Flow Immunoassay for the Detection of Plant Viruses.

ABSTRACT: Lateral flow immunoassays (LFAs) are widely used for on-site testing; however, their use for the rapid detection of plant viruses in the field is often limited by inconvenient sample preparation. Here, we present a new sampling method and a simplified dipstick LFA format for the detection and monitoring of cowpea chlorotic mottle virus (CCMV) as a model plant pathogen. The assay employs a monoclonal mouse antibody for capture and a poly-clonal rabbit antibody conjugated to 80 nm gold nanoparticles for detection. Conventional sample and conjugate pads are omitted, allowing the test strips to be dipped directly into wells containing plant extract and antibody-gold conjugate. No plastic casing was required, which could lead to a reduction in waste. It was shown that CCMV concentrations as low

SUBMITTER: Tannenberg R 

PROVIDER: S-EPMC12938336 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature