Integrated Metabolomic and Transcriptomic Analysis of Phenylpropanoid Biosynthesis in <i>Silphium perfoliatum</i>.

ABSTRACT: Silphium perfoliatum is a promising economic plant rich in bioactive secondary metabolites, yet the molecular regulation of phenylpropanoid biosynthesis across development remains unclear. To elucidate the regulatory networks underlying these metabolic processes, we integrated metabolomic and transcriptomic analyses across six developmental stages, from cotyledon to flowering. LC-MS/MS identified 1964 metabolites, with phenylpropanoids representing the largest class (601 compounds). Differential accumulation analysis showed pronounced temporal dynamics in phenylpropanoid levels, especially chlorogenic acid and its derivatives, with many compounds peaking at the flowering stage. In parallel, RNA-seq revealed 31,624 differentially expressed genes (DEGs). Functional enrichment highligh

SUBMITTER: Zhang G 

PROVIDER: S-EPMC12939584 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature