Multi-omics reveals key cell types and gene families regulating eggshell strength in chicken uteri.

ABSTRACT: Eggs represent an accessible and nutrient-dense source of high-quality animal protein, and decades of selective breeding have markedly elevated reproductive output in commercial laying hens. However, sustaining elevated productivity while improving eggshell integrity presents a critical challenge, as the molecular mechanisms of eggshell strength remain unclear. In this study, phenotypic assessment of eggshell strength was combined with single-cell transcriptomic profiling of the uterus from high- and low-strength groups, transcriptomic analysis of multiple tissues, and quantitative proteomic analysis of uterine fluid. Serum calcium and phosphorus levels did not differ significantly between groups. A single-cell atlas of the Rhode Island Red uterus was successfully generated for the first t

SUBMITTER: Zhang XK 

PROVIDER: S-EPMC12940599 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature