Genome-Wide Landscape of Position Effect Variegation in <i>Pichia pastoris</i>.

ABSTRACT: Exogenous genes are generally expressed by integration into the chromosomes of Pichia pastoris. However, systematic studies on the chromosomal position effect are lacking, and locations that are conducive to the high expression of foreign genes are rarely reported. In this study, a genomic random insertion mutagenesis library for P. pastoris was successfully constructed using the piggyBac (PB) transposon system. Through sequencing, the sequence TTAA was identified as the major recognition site of the PB transposon, which exhibited relatively high coverage on P. pastoris chromosomes, making it a valuable tool for studying position effect variegation in P. pastoris. Using the enhanced green fluorescent protein gene (eGFP) as a reporter,

SUBMITTER: Yao X 

PROVIDER: S-EPMC12941742 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature