Singularity in budding: a role for the evolutionarily conserved small GTPase Cdc42p.

ABSTRACT: The budding yeast Saccharomyces cerevisiae initiates polarized growth or budding once per cell cycle at a specific time of the cell cycle and at a specific location on the cell surface. Little is known about the molecular nature of the temporal and spatial regulatory mechanisms. It is also unclear what factors, if any, among the numerous proteins required to make a bud are involved in the determination of budding frequency. Here we describe a class of cdc42 mutants that produce multiple buds at random locations on the cell surface within one nuclear cycle. The critical mutation responsible for this phenotype affects amino acid residue 60, which is located in a domain required for GTP binding and hydrolysis. This mutation bypasses the requirement for the essential guanine-nucleotide-exchang

SUBMITTER: Caviston JP 

PROVIDER: S-EPMC129419 | biostudies-literature | 2002 Sep

REPOSITORIES: biostudies-literature