Single-Nucleus RNA Sequencing Reveals SPP1⁺ Macrophages Induce Cardiomyocyte Apoptosis to Promote Atrial Fibrillation Susceptibility.

ABSTRACT: Atrial fibrillation (AF) is closely linked to atrial remodeling, while its underlying immune mechanisms remain elusive. This study sought to investigate the role of SPP1⁺ macrophages in the development and progression of AF and further elucidate the underlying mechanisms. Single-nucleus RNA sequencing was performed on right atrial tissues from 3 patients with persistent AF and 3 with sinus rhythm (all with rheumatic valvular heart disease). The results revealed significant immune cell infiltration in AF atrial tissues, with a marked increase in the proportion of SPP1⁺ macrophages, which exhibited the strongest intercellular communication with cardiomyocytes. Phenotypic scoring indicated that apoptosis was the dominant mode of cardiomyocyte death in AF. Immunohistochem

SUBMITTER: Wang W 

PROVIDER: S-EPMC12941992 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature