An Untargeted Metabolomic Approach to Characterize the Emerging Kernel Disorder "Orange Spot" in Walnut (<i>Juglans regia</i> L.) cv. Chandler.

ABSTRACT: Background/Objectives: The physiological disorder known as "orange spot" in Juglans regia L. cv. Chandler is an emerging kernel quality issue of increasing concern for the Chilean walnut industry. Characterized by a localized orange discoloration of the embryo, its etiology remains unknown. This study aimed to determine whether orange spot is associated with bacterial infection and to elucidate the metabolic alterations underlying its development, testing the hypothesis that it results from oxidative imbalance and lipid disorganization. Methods: Untargeted metabolomic profiling and fatty acid methyl ester (FAME) analysis were applied to compare affected (orange) and unaffected (white) walnut kernels collected from a commercial orchard in Melipilla, Chile, during the 20

SUBMITTER: Hernandez I 

PROVIDER: S-EPMC12942402 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature