Investigation of Storage Conditions and Quality Control Markers for Metabolites and Lipids in Human Feces.

ABSTRACT: Background/Objectives: The stability of metabolites and lipids in feces varies depending on the storage temperature and duration. Methods: We examined the stability of various metabolites and lipids in human feces under 10 different storage conditions (room temperature for 2, 6, 24, and 48 h, 4 °C for 6, 24, and 48 h, -20 °C for 1 week, 2 weeks and 1 month) and explored markers useful for quality control of fecal samples, using metabolites and lipids that vary depending on temperature and time. Results: There was generally more variation at 4 °C than at -20 °C, and more at room temperature than at 4 °C, and variation also increased as the storage duration was extended under each temperature condition. Some metabolites and lipids were found to be unstable, even over sho

SUBMITTER: Sawada H 

PROVIDER: S-EPMC12942472 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature