Molecular Mechanisms of Temperature-Regulated Cordycepin Biosynthesis in <i>Cordyceps militaris</i>.

ABSTRACT: Cordycepin is a key active component of Cordyceps militaris, but the molecular mechanism underlying temperature-regulated biosynthesis remains unclear. In this study, Cordyceps militaris strain KN-1 was used as experimental material, with low-temperature (15 °C), control (20 °C), and high-temperature (25 °C) treatments applied during the fruiting body stage. Transcriptomics, untargeted metabolomics, weighted gene co-expression network analysis (WGCNA), and Reverse Transcription quantitative PCR (RT-qPCR) validation were integrated to elucidate the molecular mechanism of temperature-mediated cordycepin biosynthesis. The results showed that 25 °C increased fruiting body cordycepin content by 84%, while 15 °C reduced it. Transcriptomic analysis identified differentially expresse

SUBMITTER: Shao J 

PROVIDER: S-EPMC12942562 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature