Optimisation of a Caprylic Acid-Based Protocol for IgG Purification from Baboon (<i>Papio anubis</i>) Serum.

ABSTRACT: Caprylic acid (CA) fractionation of serum is a simple and cost-effective method of producing high-quality immunoglobulins. While standardised procedures exist for CA purification of IgG for various animals, no published protocol exists for baboon IgG. This study aimed to optimise an efficient protocol for purifying IgG from baboon serum using CA through a stepwise one-factor-at-a-time (OFAT) approach. The effects of serum pH, CA concentration, stirring time and intensity, dialysis buffer, and lyophilisation were evaluated based on the protein content, with SDS-PAGE profiles and albumin-globulin ratios distinguishing IgG from residual albumin. Serum at pH 5.0 with 7% CA (v/v) produced the highest yield, minimising albumin while maximising IgG content. Lower pH (4.0-4.5) and CA

SUBMITTER: Ogopotse W 

PROVIDER: S-EPMC12943085 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature