Temporal dynamics and functional annotation of transcriptome rhythmicity in HEK293T cells.

ABSTRACT: The endogenous circadian clock drives rhythmic processes in nearly all human cells; however, the temporal organization of the transcriptome in HEK293T cells, a widely used cell line, remains incompletely defined. We synchronized HEK293T cells and performed RNA sequencing at thirteen time points across a 48-hour cycle to map their transcriptome dynamics. Across the time course, principal component analysis revealed clear time point dependent separation of the global transcriptomes; however, coefficient of variation analyses indicated substantially increased divergence among biological replicates starting at T28. In addition, canonical core clock genes showed no detectable circadian rhythmicity when the analysis window extended beyond 28 hours. Genome-wide, only 785 expressed genes displayed

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12944709 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature