Identification and validation of genes encoding humoral specificity for the chemical allergen toluene diisocyanate.

ABSTRACT: A panel of hybridomas specific for different isomers of toluene diisocyanate (TDI), a cross‑linking chemical used in polyurethane production, has been previously described. These hybridomas were originally developed by researchers at the USA's National Institute for Occupational Safety and Health (NIOSH). We sought to determine the DNA sequence encoding these TDI-specific monoclonal antibodies, enabling identification of germline gene rearrangement resulting in chemical specificity as well as production of the mAbs recombinantly. B cell receptor sequencing (BCR-seq) of hybridoma RNA readily identified productive heavy and light chain antibody sequences. The productive light chains of all 7 hybridomas showed strong identity with different genomic variable (V) and joining (J) region sequence

SUBMITTER: Wisnewski AV 

PROVIDER: S-EPMC12944764 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature