Molecular Identification and Benzimidazole Resistance Analysis of Cyathostomins in Chinese Grazing Horses.

ABSTRACT: This study investigated the cyathostomin species composition, anthelmintic efficacy, and potential resistance-associated mutations in Chinese grazing horses. Fecal samples were collected from 90 adult horses. Fecal egg counts (FECs) were determined using a modified McMaster method. Third stage larvae (L3) cultured from the eggs were identified to species level by PCR amplification and sequencing of the internal transcribed spacer-2 (ITS-2). The main species included Cylicocyclus nassatus and Cylicostephanus spp. However, differences in the relative abundance of less common species, including Cyathostomum pateratum and Cylicocyclus ashworthi, suggests regional variation. Anthelmintic efficacy was assessed by a fecal egg count reduction test (FECRT) following albe

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12944989 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature