Optimization of the Quantification of Antibiotic Resistance Genes in Media from the Yangtze River Estuary.

ABSTRACT: Antibiotic resistance gene (ARG) monitoring in environmental systems increasingly relies on DNA-based molecular approaches; however, the extent to which DNA extraction strategies bias downstream resistome interpretation remains insufficiently understood. This study systematically evaluated the effects of single versus successive DNA extraction on DNA recovery, microbial community composition, and the abundance and diversity of 385 genes related to antibiotic resistance including ARGs and mobile genetic elements (MGEs) across three contrasting matrices: water, sediment, and fish intestinal tissue. Successive extraction markedly increased DNA yield and detection of functional genes in water and sediment, particularly for low-abundance and particle-associated taxa. Enhanced recovery resulted

SUBMITTER: Wu J 

PROVIDER: S-EPMC12944990 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature