New Insights into the Bioenergetic and Immunomodulatory Properties of Phospholipases A₂ from <i>Bothrops diporus</i> Venom.

ABSTRACT: Phospholipases A₂ (PLA₂s) are key mediators of the cytotoxic and inflammatory activities of snake venoms. While PLA₂ isoforms from Bothrops diporus venom have been characterized and shown to possess antimetastatic and antiangiogenic properties, their impact on mitochondrial bioenergetics and immune modulation has not yet been investigated. In this study, we examined the bioenergetic and immunomodulatory effects of B. diporus PLA₂s using integrated biochemical, metabolic, and multiplex cytokine analyses. In MDA-MB-231 breast cancer cells, pooled PLA₂s induced a dose-dependent decrease in MTT-reducing activity, increased mitochondrial ROS, caused Δψm hyperpolarization, and decreased NADH autofluorescence, collectivel

SUBMITTER: Sasovsky DJ 

PROVIDER: S-EPMC12945160 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature