Acetyl-Phosphate Dependent Protein Acetylation in Neisseria gonorrhoeae.

ABSTRACT: The disease gonorrhoea is caused by the sexually transmitted pathogen Neisseria gonorrhoeae. This bacterium is an obligate human pathogen that can survive intracellularly through the expression of specific pathogenicity determinants. Protein post-translational modifications have been shown to be involved in the regulation of gene transcription and metabolism. Here, we studied the role of non-enzymatic acetylation by acetyl-phosphate in N. gonorrhoeae. This was achieved through the deletion of pta and ackA genes from the phosphotransacetylase-acetate kinase pathway (PTA-AKA) that modulate the level of acetyl-phosphate in the cell. As predicted, more protein acetylation was observed in the ΔackA strain. Using immunoaffinity purification of acetylated peptides and LC-MS/MS we demonstrated tha

SUBMITTER: Parga EFD 

PROVIDER: S-EPMC12945711 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature