MSC-small extracellular vesicles alleviated Th2-airway inflammation by regulating the metabolism of DCs in mice.

ABSTRACT:

Background

Allergic airway inflammation is one of the chronic inflammatory diseases and is generally dominated by T helper 2 cells (Th2). Dendritic cells (DCs) are essential to mounting the Th2-mediated airway inflammation by presenting inhaled antigens to prime CD4+ T cells. Small extracellular vesicles (sEV) derived from mesenchymal stem cells (MSCs) exhibited great interest in intractable diseases. However, the effects and mechanisms of MSC-sEV on DCs in airway inflammation is still unclear.

Methods

We isolated MSC-sEV using anion-exchange chromatography. Mouse bone marrow-derived DCs (BMDCs) and human monocyte-derived DCs (moDCs) were used to study the effects of MSC-sEV on dendritic cell surface molecules and their cytokine release. Mice were sensitized with house dust

SUBMITTER: Huang LX 

PROVIDER: S-EPMC12945847 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature