Interferometric Image Scanning Microscopy for label-free imaging at 120 nm lateral resolution inside live cells.

ABSTRACT: Light microscopy remains indispensable in life sciences for visualizing cellular structures and dynamics in live specimens. Yet, conventional fluorescence imaging can suffer from phototoxicity, limited labeling efficiency, or perturbation of biological function. Label-free techniques such as interferometric scattering microscopy (iSCAT) offer a powerful alternative by detecting nanoscale structures based on their light scattering, without the need for dyes or genetic tags. iSCAT has enabled high-sensitivity detection of single proteins and viruses on clean surfaces. More recently, its application to live cells has been extended by using confocal illumination and detection, allowing suppression of out-of-focus light, yielding subcellular structures with high contrast. This development laid

SUBMITTER: Kuppers M 

PROVIDER: S-EPMC12946308 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature