Developing an Efficient System for Hybrid Rice Seed Production Using Partial Male Sterility.

ABSTRACT: Efficient production of hybrid rice seeds requires male sterility systems to overcome the challenges of self-pollination. In this study, we identified male gametic transfer defect (GTrD)5 and GTrD9 as essential genes for the process of male gamete transmission in rice. Mutations in these genes cause partial male sterility by impairing pollen tube elongation, thereby reducing the fertilisation rate. Using CRISPR/Cas9 technology, we developed gtrd5flo5 and gtrd9flo5 double mutants that combined the gtrd5 and gtrd9 genes responsible for partial male sterility with the floury endosperm (FLO)5 gene, whose defects result in seeds with an opaque endosperm, allowing us to easily differentiate between hybrid and self-pollinated seeds. This two-line hybrid system demonstrated a high rate of hybrid s

SUBMITTER: Lee SK 

PROVIDER: S-EPMC12946454 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature