Moderate Regulation of SBEIIb Gene Expression by Editing Cis-Regulatory Elements Enhances the Resistant Starch Content in Rice.

ABSTRACT: As the staple food of over half the world's population, rice with a high resistant starch (RS) content may be beneficial in the dietary prevention of diabetes and hyperlipidemia. Mutating key enzymes in starch metabolism to increase the RS content has been successful in other crops. However, there are always adverse effects, such as low grain weight, wrinkled seeds, and powdery endosperm that accompany the high RS content in rice. Here, we identified two cis-regulatory elements (CREs) related to SBEIIb by combining assay-for-transposase-accessible-chromatin sequencing, DNAase I hypersensitive site sequencing and bioinformatics analysis. The RS content of cre mutants increased by ~5.31-fold compared to the wild type, but the starch morphology, grain shape, and major agronomic traits of the

SUBMITTER: Yang Q 

PROVIDER: S-EPMC12946473 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature