An integrative spatial multi-omic workflow for unified analysis of tumor tissue.

ABSTRACT: Combining molecular profiling with imaging techniques has advanced the field of spatial biology, offering new insights into complex biological processes. Focusing on diffuse IDH-mutated glioma, this study presents a workflow for spatial multi-omics integration (SMINT) specifically combining spatial transcriptomics and spatial metabolomics. Our workflow incorporates both existing and custom-developed computational tools to enable cell segmentation and registration of spatial coordinates from both modalities to a common coordinate framework. During our investigation of cell segmentation strategies, we found that nuclei-only segmentation, while containing only 40% of segmented cell transcripts, enables accurate cell-type annotation but does not account for scenarios including delineation of m

SUBMITTER: Kriel J 

PROVIDER: S-EPMC12946760 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature