A cell line development vector strategy for improved expression of a trispecific T-cell engager in CHO.

ABSTRACT: Recent advances in trispecific antibody (trisAb) engineering offer great therapeutic potential, but achieving high product yield and quality in cell line development remains a challenge due to complex chain pairing requirements in production cell lines. In this study, three distinct expression vector configurations were evaluated for their ability to support robust, high-level expression of a structurally complex, synapse-gated trisAb T-cell engager (TriMab) in stable Chinese hamster ovary cells. Initial configurations using conventional dual heavy chain (HC) and triple light chain (LC) vectors resulted in poor pool performance characterized by delayed transfection recovery and low titers. By contrast, a redesigned strategy that reversed HC gene order and distributed LCs over separate vect

SUBMITTER: Mistry RK 

PROVIDER: S-EPMC12947573 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature