A Dual-Regulatory Mechanism of Estrogen on MTTPL-Mediated Hepatic Lipid Transport in Chickens: Involvement of <i>PPARα</i> and <i>miR-7439-3p</i>.

ABSTRACT: The liver of laying hens is the primary site for lipid synthesis and very-low-density lipoprotein (VLDL) assembly, essential for yolk formation. Unlike mammals, chickens do not require the canonical microsomal triglyceride transfer protein (MTTP) for the VLDL assembly. A newly identified gene, microsomal triglyceride transfer protein-like (MTTPL), is differently expressed during peak egg production and induced by estrogen. We demonstrated that MTTPL facilitates hepatic lipid export. Overexpression of MTTPL significantly reduced intracellular triglycerides (TG), total cholesterol (TC), and VLDL levels while increasing their extracellular concentrations, accompanied by upregulated expression of lipid-transport genes. In vivo, hepatic MTTPL knockdown impaired estro

SUBMITTER: Cao Y 

PROVIDER: S-EPMC12947693 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature