Enumeration, Phenotyping, and Clinical Associations of Tissue-Resident T Cells in the Ecto- and Endocervix of Women Attending a Colposcopy Clinic.

ABSTRACT:

Problem

Tissue-resident memory (T_RM) cells represent important immune sentinels that mount rapid recall responses to pathogens and cancers. However, there are limited data in humans on genital tract T_RM collected by clinically feasible sampling methods, limiting a full understanding of their role in immunity and clinical disease.

Method of study

We used flow cytometry and single cell RNA sequencing (scRNAseq) to characterize T cells isolated from ectocervical biopsies and endocervical cytobrushes collected from women attending a colposcopy clinic in Winnipeg, Canada.

Results

The ectocervix generally contained a higher frequency and abundance of immune cells and T-cells compared to the endocervix. CD4+ and CD8+ T_RM were more approximate

SUBMITTER: Ssemaganda A 

PROVIDER: S-EPMC12947806 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature