DNA-Guided Robust Single-Protein Electronic Readout.

ABSTRACT: Electronic transport properties of single proteins offer powerful insights into their structure and dynamics. However, the orientation variability of electrode-attached protein molecules poses a big difficulty in achieving precise electric measurements and reliable data interpretation. Here, a DNA origami-based method that promotes preferred orientations of target proteins is reported, enabling more reproducible single-molecule electrical characterization. With two orthogonal DNA aptamers installed inside a DNA origami nanocavity, a thrombin protein can be captured with a prescribed orientation between a gold substrate and a conductive AFM tip. Matrix-patterned I-V measurements reveal that bivalently anchored thrombin molecules exhibit significantly reduced conductance variability compared

SUBMITTER: Ju Z 

PROVIDER: S-EPMC12948236 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature