Efficient and Traceless Aptamer-Based Serial Selection of Naive and Early Memory CD8 T Cells for CAR T Cell Therapy.

ABSTRACT: Chimeric antigen receptor (CAR) T cell therapies have shown clinical success in cancer treatment. However, the compositions of the final products can differ substantially between patients, leading to variable treatment responses. Recent studies suggest that CAR T cells manufactured from defined T cell subsets show greater potency and persistence and improved predictability of therapeutic efficacy. Current clinical-scale selection of T cell subsets relies on antibody-based magnetic activated cell sorting, which is costly and results in suboptimal product purity and yield, presenting a significant challenge for clinical translation. Here, a high-affinity CD62L aptamer and a traceless, sequential selection system are reported for the high-yield and high-purity isolation of CD62L⁺CD8⁺ T cells

SUBMITTER: Wu AY 

PROVIDER: S-EPMC12948629 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature