Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia.

ABSTRACT: Peripheral artery disease (PAD) causes progressive arterial narrowing in the lower limbs and can advance to critical limb ischemia (CLI). Limited revascularization options highlight the need for safer, more effective therapies. Vascular multipotent stem cells (VMSCs) and adipose-derived stem cells (ADSCs) were isolated from adipose tissue, characterized phenotypically, and tested for angiogenic activity in vitro. Their therapeutic efficacy was then examined in a murine critical limb ischemia model through intramuscular transplantation, assessing limb preservation, neovascularization, and cell integration. VMSCs shared mesenchymal stem cell-like features with ADSCs and exhibited robust proliferative capacity, enabling rapid expansion to clinically relevant numbers. VMSCs also demonstrated e

SUBMITTER: Kim DY 

PROVIDER: S-EPMC12948985 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature