ZFP36 inhibits the pro-apoptotic effects of transforming growth factor β1 on mitral valve interstitial cells via the GTP-binding protein 6 pathway in mitral valve prolapse.

ABSTRACT: The objective of this work was to investigate the role of ZFP36 in mitral valve prolapse (MVP). Mitral valve and plasma were collected to assess the expression of ZFP36, transforming growth factor β (TGF-β), collagen and elastin and apoptosis rates. Mitral valve interstitial cells (MICs) were transfected with ZFP36 plasmids to observe changes in the secretion of collagen, elastin and matrix metalloproteinases (MMPs) and apoptosis rates. MICs were exposed to TGF-β1 to evaluate the changes in expression of collagen, elastin, MMPs and ZFP36 and apoptosis. Subsequently, after transfection with ZFP36 plasmid, exogenous TGF-β1 was added to the MICs, and the secretion of collagen, elastin and MMPs and apoptosis rate were re-evaluated. Finally, transcriptome and RNA immunoprecipitation (RIP) seque

SUBMITTER: Zhao M 

PROVIDER: S-EPMC12949098 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature