A dominant block to HIV-1 replication at reverse transcription in simian cells.

ABSTRACT: Although nonhuman primates are genetically close to humans, their T cells do not support productive replication of HIV-1. In contrast, HIV-1 replicates in activated human CD4(+) T cells, monocytes, and metabolically active human cells of a variety of cell types become permissive for HIV-1 replication when transduced to express CD4 and CCR5 or CXCR4. The molecular basis of this species restriction to HIV-1 replication was investigated by using African green monkey and rhesus macaque cell lines that were stably transduced to express human CD4 and CCR5. The cells supported replication of cognate viruses [simian immunodeficiency virus from African green monkeys (SIV-AGM) and macaques (SIVmac239)] but did not support replication of an R5-tropic cytopathic HIV-1. A beta-lactamase-based HIV-1 ent

SUBMITTER: Munk C 

PROVIDER: S-EPMC129785 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature