Separable stripe enhancer elements for the pair-rule gene hairy in the beetle Tribolium.

ABSTRACT: Pair-rule genes in Drosophila integrate the positional information provided by the transcription factor gradients of gap genes and set the framework for the expression of the segment polarity genes. Pair-rule genes are also expressed during the segmentation of the short germ-band embryo of the flour beetle Tribolium, although this occurs under cellular conditions that should not allow the generation of transcription factor gradients by passive diffusion. To analyse the regulation of the pair-rule gene hairy in Tribolium, we have used germline transformation with reporter gene constructs based on the piggyBac vector. We can identify an upstream fragment that drives the full expression of the eight pair-rule stripes, which are sequentially generated during embryogenesis. Further experiments

SUBMITTER: Eckert C 

PROVIDER: S-EPMC1299068 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature