A stop-EGFP transgenic mouse to detect clonal cell lineages generated by mutation.

ABSTRACT: The investigation of cell lineages and clonal organization in tissues is facilitated by techniques that allow labelling of clonal cell lineages. Here, we describe a novel transgenic mouse that allows clonal cell lineages to be traced in virtually any tissue. A green fluorescent cell lineage is generated by a random mutation at an enhanced green fluorescent protein gene that carries a premature stop codon, ensuring clonality. The transgenic system allows efficient detection of mutations and stem-cell fate mapping in the epidermis using live mice, as well as in the kidney and liver post-mortem. Cell lineages that descended from single epidermal stem cells were found to be capable of generating three adjacent corneocytes using the system, providing evidence for horizontal migration of epiderm

SUBMITTER: Ro S 

PROVIDER: S-EPMC1299129 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature