Two-fold repeated (betaalpha)4 half-barrels may provide a molecular tool for dual substrate specificity.

ABSTRACT: Some bacterial genomes contain an incomplete set of genes encoding phosphoribosyl isomerases, raising the question of whether there exists broadened substrate specificity for the missing gene products. To investigate the underlying molecular principles of this hypothesis, we have determined the crystal structure of the bifunctional enzyme PriA from Streptomyces coelicolor at 1.8 A resolution. It consists of a (betaalpha)(8)-barrel fold that is assembled by two symmetric (betaalpha)(4) half-barrels. The structure shows how its active site may catalyse the isomerization reactions of two different substrates, and we provide a plausible model of how the smaller of the two substrates could be bound in two different orientations. Our findings expand the half-barrel ancestor concept by demonstrat

SUBMITTER: Kuper J 

PROVIDER: S-EPMC1299240 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature