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Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda.

ABSTRACT: A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.

SUBMITTER: Tan YP 

PROVIDER: S-EPMC130330 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature

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Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda.

Tan Y P YP   Lin Q Q   Wang X H XH   Joshi S S   Hew C L CL   Leung K Y KY  

Infection and immunity 20021101 11

A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying viru  ...[more]

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