Static and dynamic x-ray diffraction recordings from living mammalian and amphibian skeletal muscles.

ABSTRACT: Static and time-resolved two-dimensional x-ray diffraction patterns, recorded from the living mouse diaphragm muscle, were compared with those from living frog sartorius muscle. The resting pattern of mouse muscle was similar to that of frog muscle, and consisted of actin- and myosin-based reflections with spacings basically identical to those of frog. As a notable exception, the sampling pattern of the myosin layer lines (MLL's) indicated that the mouse myofilaments were not organized into a superlattice as in frog. The intensity changes of reflections upon activation were also similar. The MLL's of both muscles were markedly weakened. Stereospecific (rigorlike) actomyosin species were not significantly populated in either muscle, as was evidenced by the 6th actin layer line (ALL), which

SUBMITTER: Iwamoto H 

PROVIDER: S-EPMC1303473 | biostudies-literature | 2003 Oct

REPOSITORIES: biostudies-literature