Transcriptional analysis of Rickettsia prowazekii invasion gene homolog (invA) during host cell infection.

ABSTRACT: An invasion gene homolog, invA, of Rickettsia prowazekii has recently been identified to encode a member of the Nudix hydrolase subfamily which acts specifically on dinucleoside oligophosphates (Np(n)N; n >/= 5), a group of cellular signaling molecules known as alarmones. InvA is thought to enhance intracellular survival by regulating stress-induced toxic nucleotide levels during rickettsial infection. To further characterize the physiological function of InvA, the gene expression pattern during various stages of rickettsial intracellular growth was investigated. Using semiquantitative reverse transcription-PCR (RT-PCR) and real-time fluorescent probe-based quantitative RT-PCR, a differential expression profile of invA during rickettsial host cell infection was examined. The invA transcrip

SUBMITTER: Gaywee J 

PROVIDER: S-EPMC130406 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature