Quantitative imaging of lymphocyte membrane protein reorganization and signaling.

ABSTRACT: Changes in membrane protein localization are critical to establishing cell polarity and regulating cell signaling. Fluorescence microscopy of labeled proteins allows visualization of these changes, but quantitative analysis is needed to study this aspect of cell signaling in full mechanistic detail. We have developed a novel approach for quantitative assessment of membrane protein redistribution based on four-dimensional video microscopy of fluorescently labeled proteins. Our analytic system provides robust automated methods for cell surface reconstruction, cell shape tracking, cell-surface distance measurement, and cluster formation analysis. These methods permit statistical analyses and testing of mechanistic hypotheses regarding cell signaling. We have used this approach to measure anti

SUBMITTER: Kasson PM 

PROVIDER: S-EPMC1305035 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature