Assembly of lipoprotein particles containing apolipoprotein-B: structural model for the nascent lipoprotein particle.

ABSTRACT: Apolipoprotein B (apoB) is the major protein component of large lipoprotein particles that transport lipids and cholesterol. We have developed a detailed model of the first 1000 residues of apoB using standard sequence alignment programs (ClustalW and MACAW) and the MODELLER6 package for three-dimensional homology modeling. The validity of the apoB model was supported by conservation of disulfide bonds, location of all proline residues in turns and loops, and conservation of the hydrophobic faces of the two C-terminal amphipathic beta-sheets, betaA (residues 600-763) and betaB (residues 780-1000). This model suggests a lipid-pocket mechanism for initiation of lipoprotein particle assembly. In a previous model we suggested that microsomal triglyceride transfer protein might play a structura

SUBMITTER: Richardson PE 

PROVIDER: S-EPMC1305374 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature