Untangling the wires: a strategy to trace functional interactions in signaling and gene networks.

ABSTRACT: Emerging technologies have enabled the acquisition of large genomics and proteomics data sets. However, current methodologies for analysis do not permit interpretation of the data in ways that unravel cellular networking. We propose a quantitative method for determining functional interactions in cellular signaling and gene networks. It can be used to explore cell systems at a mechanistic level or applied within a "modular" framework, which dramatically decreases the number of variables to be assayed. This method is based on a mathematical derivation that demonstrates how the topology and strength of network connections can be retrieved from experimentally measured network responses to successive perturbations of all modules. Importantly, our analysis can reveal functional interactions eve

SUBMITTER: Kholodenko BN 

PROVIDER: S-EPMC130547 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature