A consistent experimental and modeling approach to light-scattering studies of protein-protein interactions in solution.

ABSTRACT: The osmotic second virial coefficient, B(2), obtained by light scattering from protein solutions has two principal components: the Donnan contribution and a contribution due to protein-protein interactions in the limit of infinite dilution. The Donnan contribution accounts for electroneutrality in a multicomponent solution of (poly)electrolytes. The importance of distinguishing this ideal contribution to B(2) is emphasized, thereby allowing us to model the interaction part of B(2) by molecular computations. The model for protein-protein interactions that we use here extends earlier work (Neal et al., 1998) by accounting for long-range electrostatic interactions and the specific hydration of the protein by strongly associated water molecules. Our model predictions are compared with measurem

SUBMITTER: Asthagiri D 

PROVIDER: S-EPMC1305478 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature