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The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts.

ABSTRACT: The 3.31-Mb genome sequence of the intracellular pathogen and potential bioterrorism agent, Brucella suis, was determined. Comparison of B. suis with Brucella melitensis has defined a finite set of differences that could be responsible for the differences in virulence and host preference between these organisms, and indicates that phage have played a significant role in their divergence. Analysis of the B. suis genome reveals transport and metabolic capabilities akin to soil/plant-associated bacteria. Extensive gene synteny between B. suis chromosome 1 and the genome of the plant symbiont Mesorhizobium loti emphasizes the similarity between this animal pathogen and plant pathogens and symbionts. A limited repertoire of genes homologous to known bacterial virulence factors were identified.

SUBMITTER: Paulsen IT

PROVIDER: S-EPMC130601 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature

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The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts.

Paulsen Ian T IT Seshadri Rekha R Nelson Karen E KE Eisen Jonathan A JA Heidelberg John F JF Read Timothy D TD Dodson Robert J RJ Umayam Lowell L Brinkac Lauren M LM Beanan Maureen J MJ Daugherty Sean C SC Deboy Robert T RT Durkin A Scott AS Kolonay James F JF Madupu Ramana R Nelson William C WC Ayodeji Bola B Kraul Margaret M Shetty Jyoti J Malek Joel J Van Aken Susan E SE Riedmuller Steven S Tettelin Herve H Gill Steven R SR White Owen O Salzberg Steven L SL Hoover David L DL Lindler Luther E LE Halling Shirley M SM Boyle Stephen M SM Fraser Claire M CM

Proceedings of the National Academy of Sciences of the United States of America 20020923 20

The 3.31-Mb genome sequence of the intracellular pathogen and potential bioterrorism agent, Brucella suis, was determined. Comparison of B. suis with Brucella melitensis has defined a finite set of differences that could be responsible for the differences in virulence and host preference between these organisms, and indicates that phage have played a significant role in their divergence. Analysis of the B. suis genome reveals transport and metabolic capabilities akin to soil/plant-associated bac ...[more]

PMID: 12271122

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Project description:BackgroundSwine brucellosis caused by B. suis biovar 2 is an emergent disease in domestic pigs in Europe. The emergence of this pathogen has been linked to the increase of extensive pig farms and the high density of infected wild boars (Sus scrofa). In Portugal and Spain, the majority of strains share specific molecular characteristics, which allowed establishing an Iberian clonal lineage. However, several strains isolated from wild boars in the North-East region of Spain are similar to strains isolated in different Central European countries.ResultsComparative analysis of five newly fully sequenced B. suis biovar 2 strains belonging to the main circulating clones in Iberian Peninsula, with publicly available Brucella spp. genomes, revealed that strains from Iberian clonal lineage share 74% similarity with those reference genomes. Besides the 210 kb translocation event present in all biovar 2 strains, an inversion with 944 kb was presented in chromosome I of strains from the Iberian clone. At left and right crossover points, the inversion disrupted a TRAP dicarboxylate transporter, DctM subunit, and an integral membrane protein TerC. The gene dctM is well conserved in Brucella spp. except in strains from the Iberian clonal lineage. Intraspecies comparative analysis also exposed a number of biovar-, haplotype- and strain-specific insertion-deletion (INDELs) events and single nucleotide polymorphisms (SNPs) that could explain differences in virulence and host specificities. Most discriminative mutations were associated to membrane related molecules (29%) and enzymes involved in catabolism processes (20%). Molecular identification of both B. suis biovar 2 clonal lineages could be easily achieved using the target-PCR procedures established in this work for the evaluated INDELs.ConclusionWhole-genome analyses supports that the B. suis biovar 2 Iberian clonal lineage evolved from the Central-European lineage and suggests that the genomic specialization of this pathogen in the Iberian Peninsula is independent of a specific genomic event(s), but instead driven by allopatric speciation, resulting in the establishment of a new ecovar.

Dataset Information

The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts.

Publications

The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts.

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