Improved molecular weight-based processing of intact proteins for interrogation by quadrupole-enhanced FT MS/MS.

ABSTRACT: Complete coverage of protein primary structure is demonstrated for 37 yeast protein forms between 6 and 30 kDa in an improved platform for Top Down mass spectrometry (MS). Tandem mass spectrometry (MS/MS) for protein identification with 100% sequence coverage is achieved in a highly automated fashion with 15-300-fold less sample amounts than an initial report of a proteome fractionation approach employing preparative gel electrophoresis with an acid-labile surfactant to facilitate reversed phase separation in a second dimension. Using a quadrupole-enhanced Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FTICRMS) improves the dynamic range for protein detection by approximately 50-fold and MS/MS by approximately 30-fold. The technology development illustrated here typifies an a

SUBMITTER: Du Y 

PROVIDER: S-EPMC1307227 | biostudies-literature | 2004 Jul-Aug

REPOSITORIES: biostudies-literature