Identification of Bartonella bacilliformis genotypes and their relevance to epidemiological investigations of human bartonellosis.

ABSTRACT: Genotypic diversity among 26 isolates of Bartonella bacilliformis obtained from different areas of Peru, and at different times, was assessed by comparison of DNA sequences derived from 16S-23S ribosomal DNA intergenic spacer regions (ISR) and a citrate synthase gene (gltA) fragment and by amplified fragment length polymorphism (AFLP) analysis. gltA comparison divided the isolates into two groups, whereas ISR comparison revealed six sequences. AFLP analysis using a selective primer delineated five profiles that correlated well with those obtained by sequence comparison. Combination of all three data sets divided the isolates into six genotypes. One of these genotypes was common to isolates collected from a large area in western Peru that corresponded to the region of endemicity for bartone

SUBMITTER: Birtles RJ 

PROVIDER: S-EPMC130851 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature