Mapping and characterization of the amplicon near APOA2 in 1q23 in human sarcomas by FISH and array CGH.

ABSTRACT:

Background

Amplification of the q21-q23 region on chromosome 1 is frequently found in sarcomas and a variety of other solid tumours. Previous analyses of sarcomas have indicated the presence of at least two separate amplicons within this region, one located in 1q21 and one located near the apolipoprotein A-II (APOA2) gene in 1q23. In this study we have mapped and characterized the amplicon in 1q23 in more detail.

Results

We have used fluorescence in situ hybridisation (FISH) and microarray-based comparative genomic hybridisation (array CGH) to map and define the borders of the amplicon in 10 sarcomas. A subregion of approximately 800 kb was identified as the core of the amplicon. The amplification patterns of nine possible candidate target genes located to this subregion wer

SUBMITTER: Kresse SH 

PROVIDER: S-EPMC1308856 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature