SSH adequacy to preimplantation mammalian development: scarce specific transcripts cloning despite irregular normalisation.

ABSTRACT:

Background

SSH has emerged as a widely used technology to identify genes that are differentially regulated between two biological situations. Because it includes a normalisation step, it is used for preference to clone low abundance differentially expressed transcripts. It does not require previous sequence knowledge and may start from PCR amplified cDNAs. It is thus particularly well suited to biological situations where specific genes are expressed and tiny amounts of RNA are available. This is the case during early mammalian embryo development. In this field, few differentially expressed genes have been characterized from SSH libraries, but an overall assessment of the quality of SSH libraries is still required. Because we are interested in the more systematic establishment of S

SUBMITTER: Bui LC 

PROVIDER: S-EPMC1312315 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature