Premature condensation induces breaks at the interface of early and late replicating chromosome bands bearing common fragile sites.

ABSTRACT: Various studies suggest a tight relationship between chromosome rearrangements driving tumor progression and breaks at loci called common fragile sites. Most of these sites are induced after perturbation of the replication dynamics, notably by aphidicolin treatment. We have mapped the majority of these sites to the interface of R and G bands, which calls into question the previous assignment of aphidicolin-sensitive sites to R bands. This observation suggests that most of them correspond to loci that ensure the transition between early and late replicating domains. We show that calyculin A, which triggers chromosome condensation at any phase of the cell cycle but does not markedly impair replication, induces damage in the chromosomes of human lymphocytes treated in G(2) but not in G(1) pha

SUBMITTER: El Achkar E 

PROVIDER: S-EPMC1312387 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature