SiteFind: a software tool for introducing a restriction site as a marker for successful site-directed mutagenesis.

ABSTRACT:

Background

Site-directed mutagenesis is a widely-used technique for introducing mutations into a particular DNA sequence, often with the goal of creating a point mutation in the corresponding amino acid sequence but otherwise leaving the overall sequence undisturbed. However, this method provides no means for verifying its success other than sequencing the putative mutant construct: This can quickly become an expensive method for screening for successful mutations. An alternative to sequencing is to simultaneously introduce a restriction site near the point mutation in manner such that the restriction site has no effect on the translated amino acid sequence. Thus, the novel restriction site can be used as a marker for successful mutation which can be quickly and easily assessed. Ho

SUBMITTER: Evans PM 

PROVIDER: S-EPMC1314889 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature